We'll show you how to best prepare for a testosterone test to get the most accurate and reliable results. When it comes to determining your testosterone levels, proper preparation is crucial for accurate and reliable results. In this article, we offer a simple guide to help you prepare optimally for your testosterone test.
It's important that you take certain precautions and avoid certain activities to ensure your test results are as accurate as possible. Here are the essential do's and don'ts to follow to get the best insight into your hormone levels.
Testosterone plays a central role in many physiological processes in the body, including muscle mass, bone strength, energy levels, and libido ( Harvard Health, 2023 ). Whether you are experiencing actual symptoms of low testosterone or simply want to monitor your health, it is important to perform the test correctly. Preparation for the test begins several days beforehand and involves various aspects of your lifestyle and daily routine.
What is a testosterone blood test?
A testosterone blood test is used to measure the concentration of testosterone in the bloodstream. Testosterone is produced in significantly smaller amounts by the testes in men and by the ovaries in women ( Vierhapper et al., 1997 ) when the brain sends them a signal to produce it ( Acevedo-Rodriguez et al., 2018 ). It is then released into the bloodstream and travels to various organs and tissues, where it exerts its effects.
Testosterone exists in the blood in two forms:
- Bound testosterone: The portion of testosterone bound to sex hormone-binding globulin (SHBG), albumin, and other blood proteins .
- Free testosterone: Testosterone not bound to serum proteins.
Both free testosterone and testosterone bound to the blood protein albumin are collectively referred to as bioavailable testosterone ( Krakowsky & Grober, 2015 ). The bond between testosterone and albumin is relatively weak, allowing it to dissociate easily. SHBG-bound testosterone is inactive, while bioavailable testosterone is considered active because it is the portion of testosterone that can be used by tissues as needed ( Goldman et al., 2017 ).
The classic testosterone blood test measures the amount of free testosterone, bound testosterone, and bioavailable testosterone, also collectively referred to as total testosterone . However, a direct measurement of free testosterone alone is often unnecessary, as a relatively accurate estimate can be achieved using a formula. For this estimate, it is sufficient to determine total testosterone as well as the two blood proteins SHBG and albumin ( Vermeulen et al., 1999 ).
It is primarily total testosterone and free testosterone that are medically checked. This can help to identify a possible cause of erectile dysfunction, low libido, and other sexual problems ( Hintikka et al., 2009 ).
When you should consider a testosterone test
In men, normal testosterone levels range from 300 to 1,000 nanograms per deciliter (ng/dL) ( Zhu et al., 2022 ), while in women, healthy testosterone levels range from 15 to 70 ng/dL. Testosterone levels are known to decrease over the course of a person's life, but they can also fluctuate, particularly due to underlying health problems ( Kelly & Jones, 2015 ).
Symptoms that men with low testosterone may experience include:
- Low libido ( Hintikka et al., 2009 )
- Erectile dysfunction ( Wang et al., 2000 )
- Frequent fatigue ( Wang et al., 2000 )
- Lack of motivation ( Zitzmann, 2020 )
- Anxiety and depression ( Hintikka et al., 2009 )
- Concentration difficulties ( Smith et al., 2018 )
- Sleep problems ( Agrawal et al., 2022 )
- Hot flashes ( Harvard Health, 2021 )
- Increase in body fat ( Wittert et al., 2003 )
- Loss of bone density ( Fink et al., 2006 )
- Decrease in muscle mass ( Bhasin et al., 1997 )
- Hair loss ( Jerjen et al., 2021 )
- Anemia (low red blood cell count) ( Pencina et al., 2023 )
- Enlarged breasts (gynecomastia) ( Ersöz et al., 2002 )
If you notice these symptoms, a test may be useful to clarify the cause and, if necessary, initiate appropriate treatment.
What you should do before your test
Choosing the right time
Testosterone levels are typically highest in the morning. Therefore, it is important to schedule the test early in the day, ideally between 7 and 10 a.m. ( Brambilla et al., 2009 ). This ensures that your testosterone levels are at their highest and that the results are as accurate as possible. Furthermore, subsequent tests should always be performed at the same time to guarantee the best possible comparability ( Collier et al., 2010 ).
If you would like to learn more about testosterone fluctuations and the so-called circadian rhythm, please read our article " Hormonal Fluctuations: Understanding the Highs and Lows of Testosterone Levels ".
Communicate with your doctor
Inform your doctor about all medications and supplements you are taking. Some substances can affect your testosterone levels and lead to inaccurate results ( Coluzzi et al., 2018 ). These include prescription medications, over-the-counter drugs, herbal supplements, and vitamins. Clear communication with your doctor will help minimize potential interference and ensure your test results are as accurate as possible. Your doctor can also give you specific instructions on whether you should discontinue certain medications or supplements before the test.
Stay hydrated
Drink plenty of water before the test to ensure you are well hydrated. Good hydration can help obtain more accurate blood test results. Dehydration can affect blood composition and distort test results.
Make sure you get enough sleep.
Make sure you get at least 7-8 hours of sleep the night before the test. Lack of sleep can negatively affect your testosterone levels, as the body regulates and renews hormones during sleep. A good night's rest is therefore important to ensure your test results are as accurate as possible. Try developing a relaxing evening routine to help you sleep better ( Ukraintseva et al., 2018 ).
What you should avoid before your test
Avoid excessive stress
Try to minimize stress before the test, as high stress levels can affect your hormone levels, including testosterone. Stress can increase cortisol levels, which in turn can inhibit testosterone production ( Xiong et al., 2021 ). Relaxation techniques such as meditation, deep breathing, or yoga can be helpful to calm you and lower your stress levels ( Gothe et al., 2016 ). Ideally, plan relaxing activities the evening before the test to ensure you are in the calmest and most balanced state possible.
Avoid strenuous training
Avoid strenuous exercise the day before your test. Intense physical activity can temporarily alter your testosterone levels and lead to inaccurate results. Intense exercise can cause testosterone levels to rise or fall in the short term, which can distort the measurement. Stick to light activities such as walking or gentle stretching to ensure your testosterone levels are within the normal range during the test ( Riachy et al., 2020 ).
Avoid alcohol and caffeine
Avoid alcohol and caffeine for 24 hours before your test. Both substances can affect your hormone levels and should be avoided to ensure accurate test results. Alcohol can suppress testosterone production and disrupt hormone balance ( SJ Smith et al., 2023 ), while caffeine can increase cortisol levels ( Lovallo et al., 2005 ), which in turn can affect testosterone levels. By avoiding alcohol and caffeinated beverages such as coffee, tea, and certain soft drinks, you will help ensure your testosterone levels are measured as accurately as possible. Drink water or decaffeinated beverages instead to stay hydrated and keep your hormone levels stable.
Avoid smoking
Smoking can affect your testosterone levels. It is advisable to refrain from smoking before the test to obtain the most accurate results. The chemicals in tobacco can disrupt the hormonal balance and distort your testosterone levels ( Zhao et al., 2016 ).
Our conclusion, your knowledge
By following these recommendations, you can ensure that your testosterone levels are measured as accurately as possible. A well-prepared testing procedure allows for a more precise diagnosis and therefore the best possible treatment. By following these tips, you actively contribute to your doctor gaining a clear picture of your hormonal status and being able to offer you the best possible support. Remember that small changes in your preparation can make a big difference in the accuracy of your test results.
